WebWilliam Grupp Heather Gurtowski April Haberstroh Andrea t Cecile Laurie Hansen Janet Hanus Kristen Harrop Page 52 text: Steve Kopff Steve Koppel Charles Kaiser Besty Kammerer Keith Kargl Roland Katz A Steve Kaufman Andrea Kaye Robin Kazen Kelly Kempner Ted Klinck Kathy Knapp Cindy Kocimski Mitchell Kolesar O Web目的观察小干扰RNA(siRNA)对人淀粉样前体蛋白(APP)基因的沉默作用.方法将设计好的一对寡核苷酸在细胞内源性地转录成小发夹RNA(shRNA),同时构建了携带2个APP亚型的增强型绿色荧光蛋白(EGFP)的GFP-APP重组质粒;利用EGFP作为报告基因,在COS-7细胞内通过共转染siRNA 表达质粒和GFP-APP重组质粒来观察沉默效应.结果 ...
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